Evaluation of PBMC Isolation Methods and the Impact of Cryopreservation

Immunophenotyping is key to understanding immunological responses in clinical trials. To minimize batch effects, we compared three PBMC isolation methods—Ficoll separation, SepMate, and CPT tubes—using samples from three healthy donors, evaluating yield, viability, and variability pre- and post-thaw.

• Understanding different PBMC isolation methods (Ficoll, SepMate, CPT tubes) and their results in terms of cell viability and recovery.

• Understanding these factors is critical for optimizing PBMC isolation and cryopreservation protocols to ensure robust, reproducible immunophenotyping in clinical studies.

• Valuable insights into how isolation and cryopreservation methods influence immune cell stability, improving flow cytometry-based analysis in longitudinal and global clinical trials.