CYTO 2026 Poster P011: Development of Robust Evaluation of T Cell Subsets with T-Cell Activation In a 3D Tumor–PBMC Co-Culture Model Enables Reliable Phenotyping and Cytotoxicity Assessment

Established optimized culture conditions, tumor dissociation, and staining protocols for accurate flow cytometry analysis.
How using anti-CD3/CD28 stimulation and refined methods, we achieved reliable T cell activation and quantification of tumor killing, with upregulation of activation markers (CD69, CD25) and distinct CD4+ and CD8+ recruitment in the 3D system.
A platform enables precise measurement of T cell activation, proliferation, cytokine release, and tumor cell death, and serves as a standardized benchmark for preclinical IO and T cell engager screening.
The multiplex platform that allows flexible customization of selective cytokine analysis panels.

CYTO 2026 Poster #P011